National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Knowledge of genetic diversity and relationship among breeding material at molecular level has significant impact on safflower improvement. Genetic diversity of eight parental genotypes of safflower (Carthamus tinctorius L.) was investigated at DNA level with Random Amplified Polymorphic DNA (RAPD) procedure. Forty RAPD primers generated 943 alleles, out of which 679 alleles were found polymorphic, resulting 72.00 per cent polymorphism. The similarity coefficient ranged from 0.42 to 0.76 with an average of 0.59, thus suggesting considerable genetic variation among the safflower genotypes studied. Further, the dendrogram generated by UPGMA cluster analysis based on Jaccard’s similarity coefficient grouped eight parental genotypes into two clusters viz., cluster A and cluster B. Maximum number (7 out of 8) cultivars fell in cluster A. Cluster A was further sub divided in sub cluster A1 and A2. Least similarity of (0.42) demonstrated by GMU-2720 and EC-757665 followed by PBN-96 and EC-757665 as well as by NARI-6 and EC-757665. Genotypes, GMU-2720 and PBNS-12, GMU-1339 and PBNS-12 were found to be (0.76) per cent similar based on molecular analysis, but could not consider as duplicate. It may be due to narrowness of the genetic base of widely grown safflower and the results may be different if large number of RAPD primers may use in the study. Our result suggests that, RAPD molecular markers can detect high polymorphism and efficient for assuring genetic diversity and relatedness and also aid in selection of diverse outstanding lines to be used in future breeding programs of safflower.
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