National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Genetic diversity assessment is necessary to help tackle the threats of environmental fluctuations and for the effective exploitation of genetic resources in breeding program. Recent advancement in the field of molecular markers has made the genetic characterization of genotypes rapid, reliable and reproducible. In the present investigation, we have characterized 49 wheat genotypes at molecular level using 52 SSR primers (including Yr specific primers). 27 polymorphic SSR markers were dispersed over the AABBDD wheat genome, a total of 102 alleles were detected with allele range of 1 to 6. Polymorphism information content (PIC) values calculated to assess the informativeness of each marker ranged from 0.11 to 0.95 and there is significant that 5 out of 27 SSR loci, namely Xpsp 3000, Xwgp249, Wmc198, csLV34, Xgwm301 revealed PIC values above 0.70, can be considered highly useful for differentiation of wheat genotypes. The UPGMA cluster tree analysis led to the grouping of 49 wheat genotypes in two major clusters and nine sub clusters. Cluster pattern revealed that, sub-cluster six was the largest consisting maximum number of twelve genotypes. Our results suggested that the classification based on genotypic markers of these wheat genotypes would be useful for selection of varieties for wheat improvement program.
Citations |
 |
 |
 |
 |
