National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Gold nanoparticles (GNPs) an emerging part of nanomedicine holds great promise for revolutionizing medical treatments and therapies in areas such as imaging, drug delivery, faster diagnosis, and tissue regeneration, as well as the development of new medical products. But their safety assessment is a major concern. As body system reacts in a different way than isolated cell line than artificial environment, so in the present experiment pathomorphological assessment ofsub-acute dermal toxicity of green synthesized GNPs and coating with Morinda citrifolia fruit extract (McFE) was undertaken. Gold nanoparticles were synthesized by biological reduction of chloroauric acid with Azadirachta indica (neem) 5% leaf extract. The nanoparticles characterized and were confirmed by colour changes from pale yellow to ruby red. These nanoparticles were coated with McFE.For toxicity evaluation.50 Wistar rats of either sex were assigned to five groups. One group was considered healthy, three groups received dermal application of combination of 1 mg/kg GNPs with 150mg/kg McFE (Low dose), 2mg/kg GNPs with 300mg/kg McFE (Medium dose) and 4 mg/kg GNPs with 600mg/kg McFE (High dose) dermal application for 28 days. The last group was considered as satellite group which received 8 mg/kg GNPs with 1200 mg/kg McFE and observed for extra 14 day beyond trial period for checking post withdrawal effect. Grossly mild hemorrhages, congestion and architectural alteration were observed in liver and kidney treated with highest dose. However the histopathological examination of skin revealed no alteration in skin architecture but minimal alterations were observed in liver and kidney where major changes like dilatation of central vein and sinusoidal space, multifocal necrobiotic changes and focal fatty change in liver and cystic degeneration and hyaline cast in lumen of kidney noticed indicating proper absorption and excretion of GNPs from body.
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