National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Stool is one of the most difficult laboratory samples to be handled especially for recovery of intestinal parasites. The preservation of stool parasites faces a lot of difficulties in laboratory and the use of powerful preservatives leads to a better result. This study describes the efficacy of three stool preservatives for the stool parasites and they are 10% formalin, low viscosity polyvinyl alcohol and sodium acetate acetic acid formalin and compare them using fresh stool which were positive for intestinal parasites. Stool samples positive for an intestinal parasite by microscopy were taken and the outcome was compared after preserving the sample with each of the 3 preservatives and observed after 1 month period in relation to their morphology and staining properties. Of all the 3 preservatives, morphologic identification was more satisfactory with 10% formalin and least with low viscosity polyvinyl alcohol. However, all didn’t give much satisfying results with a permanent stain like trichrome though the best result both in terms of morphological identification and proper taking up of the stain was seen in those preserved with 10% formalin followed by SAF and least in those with LV-PVA. Using stool preservative is another viable alternative to using fresh stool and it helps to retain the morphology to a certain extent.
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