National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present research was undertaken with an aim to isolate and characterize antifungal compounds from actinomycetes and optimization of cultural characteristics for production of bioactive metabolite by actinomycetes. Actinomycetes isolate ACITM-1 was further cultivated in different conditions fermentation in order to optimize biosynthetic process of antibiotic production including: different pH values, different temperatures, different carbon and nitrogen sources were fully investigates. Actinomycetes isolate ACITM-1 was isolated from soil of Gwalior Chambal region, MP, (India) was found to show significant antifungal activity against phytopathogens of soybean crop. Based on physiological, biochemical characteristics the isolate was identified as Streptomyces chilikensis ACITM-1. This strain was further cultivated in different conditions in order to optimize the growth of secondary metabolite production. The antimicrobial activity and biomass production was highest when isolates were cultivated in media having starch as a carbon source. Peptone and potassium nitrate were found best nitrogen source for the highest antifungal activity and biomass production. Form the experiment it was observed that antifungal activity was found maximum with starch, peptone and potassium nitrate in 96 hours at 30º C.
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