National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this study an attempt was taken to determine the spontaneous haemolytic activity 50 % (SH50%) of healthy catla (Catla catla) fingerlings serum with respect to its characteristics features such as optimal temperature, ability for lysing red blood cells (RBCs) from various animals, heat sensitivity, storage temperature and effect of different agents like EDTA, EGTA. In addition to these, complement binding factors such as Zymosan, Lipopolysacharide were also tested for SH 50% activity. Furthermore, the effect of bacterial culture was also determined on SH 50% activity of catla sera. The most advantageous temperature for rabbit RBCs lysis was determined as 20 °C for catla serum. The normal catla serum was found to be heat sensitive and it was obtained as more than 60 °C. The haemolytic activity of catla sera showed reasonable activity after short term storage at -20 °C and good activity after six months storage at -80 °C. Furthermore, the chemical effects of ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) and Ethylenediaminetetra acetic acid (EDTA) on SH 50 % activity was found to be opposite in action, inhibition of SH 50 % was obtained with EGTA whereas enhanced SH 50% activity with EDTA. Also, the inhibitory effects of Zymosan, bacterial LPS and Aeromonas hydrophila culture were detected for haemolytic activity of catla sera.
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