Urinary Tract Infections are among the most common bacterial infections and account for a significant part of the workload in clinical microbiology laboratories. The rate of yield is almost same for all media i.e., Blood Agar, MacConkey gar, C.L.E.D Agar with Andrade’s indicator and Hichrome UTI Agar. The results obtained after various specific biochemical tests and other techniques, were correlated correctly with the presumptive identification results by Hicrome UTI Agar. Presumptive identification of isolates is a task that requires a great deal of experience when using traditional media like blood agar and Mac Conkey agar. On chromogenic media, this is easier, thus requiring less training. Thus, the use of chromogenic media may improve the quality of urine culture by contributing to a more uniform interpretation of urine culture plates by the different personnel engaged in this task at the laboratory. Blood agar should continue to be a part of the urine culture workup, as isolation and discrimination of different Gram-positive bacteria species are much easier with this medium. As many of the extra tests for bacterial identification associated with conventional culture methods were no longer required, chromogenic medium substantially reduced the laboratory workload. For detection of mixed bacterial growth Hichrome UTI Agar precedes the other media. The excessive use of Blood agar can be minimised in 24 hr working microbiology laboratories. Therefore Hichrome UTI Agar or C.L.E.D Agar with Andrade’s indicator is advisable as primary plating media. HiChrome UTI agar was found to be more useful as primary urine culture medium in both higher rate of isolation and presumptive identification of uropathogens in comparison to conventional media. Its inherent characteristics in demonstrating polymicrobial growth and ease of rapid identification by distinct colony colour are unique. Improved detection of mixed cultures may help to identify contaminated specimens and therefore lead to a reduction in the prescription of unnecessary antibiotics. The overall findings of this study suggests and reinforced that HiCrome UTI agar offers an excellent and time saving method for the reliable identification of most of the uropathogens and differentiation of mixed bacterial cultures in primary culture plate as opposed to conventional culture system. This study suggests and reinforced that HiCrome UTI agar offers an excellent and time saving method for the reliable identification of most of the uropathogens and differentiation of mixed bacterial cultures in primary culture plate as opposed to conventional culture system. It has the potential to streamline urine culture processing in a meaningful way, such as reducing technologist workload, improving result turnaround times which together all have considerable laboratory impact.

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