National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was conducted to develop a protocol for rapid plant regeneration of apple. The leaf explants of apple (Pyrus malus L.) cultivar viz. Gold Delicious was cultured for plant regeneration. The explants were cultured on MS media supplemented with different concentrations and combinations of BA with IBA and NAA as well as Kinetin with NAA. Maximum induction (80%) of leaf explants of Golden Delicious was observed in medium containing BA + NAA (1.0+0.5 mg/L). MS medium supplemented with different concentrations and combinations of BA, NAA and KIN were also employed for shoot regeneration. MS medium comprises BA (2.0 mg/L) and NAA (0.02 mg/L) in a particular concentration was best for maximum shoot regeneration from leaf calli whereas MS medium containing BA (2.0 mg/L) + NAA (0.02 mg/L) + GA3 (0.4 mg/L) was best for maximum shoot elongation. The micro shoots were successfully rooted on half strength MS medium supplemented with IBA (1.0 mg/L) alone and 96.66% rooted plantlets were obtained using the medium. The in vitro plantlets were acclimatized in jiffy pots after rooting and established in the soil. The protocol of in vitro propagation, presented here is suitable and efficient for cost effective as well as timely production of plantlets for apple (Pyrus malus).
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