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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:8, August, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(8): 326-335
DOI: https://doi.org/10.20546/ijcmas.2019.808.037


In-Vitro Evaluation of Bio-control Agents against Alternaria Leaf blight of Sunflower and Vascular Wilt of Beans
Bushra Rasool, Baby Summuna*, T.A. Shah and Umbarkar Pooja
SKUAST- Kashmir, India
*Corresponding author
Abstract:

Four fungal bio-control agents, viz., Trichoderma harzianum, T. viride, Aspergillus spp. and Tricothecium spp. were evaluated in vitro through dual culture technique against Alternaria helianthi, the causative agent of leaf blight of sunflower and Fusarium oxyporum f. sp. phaseoli, the causative agent of vascular wilt of beans. The observations were taken after fourth and seventh day for both the pathogens. After fourth day, observations recorded in case of A. helianthi, the minimum colony diameter (0 cm) and maximum inhibition percentage over control (100%) were observed in Trichoderma harzianum and T. viride which were statistically at par with Aspergillus spp. and Tricothecium spp. while after seventh day, the result was more or less similar. The minimum colony diameter (0 cm) of pathogen were observed in Trichoderma harzianum and T. viride with maximum inhibition per cent (100%) over control, which differed statistically from Aspergillus spp.. Tricothecium spp. showed least effectiveness against the target pathogen showing colony diameter (2.3 cm) and inhibition (23.33%) after fourth day and colony diameter (4.5 cm) and inhibition (35.71%) after seventh day over control. After fourth day, the observations taken in case of F. oxyporum f. sp. phaseoli, the minimum colony diameter (1.6 cm) and maximum inhibition per cent over control (77.14%) were observed in T. harzianum which were statistically at par with T. viride, Aspergillus spp. and Tricothecium spp. After seventh day the result was more or less similar. The minimum colony diameter (1.2 cm) of pathogen was observed in T. harzianum with maximum inhibition percentage (86.66%) over control, which differed statistically from T. viride and Aspergillus spp. Tricothecium spp. showed least effectiveness against the target pathogen showing colony diameter (3.5 cm) and inhibition per cent (50%) after fourth day and colony diameter (3.0 cm) and inhibition per cent (66.66%) after seventh day over control.


Keywords: Bio-control agents, Beans, Fungus, Sunflower

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How to cite this article:

Bushra Rasool, Baby Summuna, T.A. Shah and Umbarkar Pooja. 2019. In-Vitro Evaluation of Bio-control Agents against Alternaria Leaf blight of Sunflower and Vascular Wilt of Beans.Int.J.Curr.Microbiol.App.Sci. 8(8): 326-335. doi: https://doi.org/10.20546/ijcmas.2019.808.037
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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