National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The cytotoxicity and virus titration of chitosan nanoparticles encapsulated live PPR virus vaccine (PPRV-CS-NPs) was analyzed in this study. Live attenuated PPR virus vaccine (AR-87) was encapsulated in chitosan nanoparticles using ionic gelation technique. The PPRV-CS-NPs were tested for cytotoxicity study in Vero cells with different concentrations (1-40ïg).The cell viability was more than 80 per cent with all the concentration of the PPRV-CS-NPs tested. This indicates that the PPRV-CS-NPs are not toxic even in concentration higher than the required vaccine dose. The viability of PPRV (AR-87) in the PPRV-CS-NPs were tested by releasing the virus from nanoparticles by trypsin digestion. The virus titration was carried out in Vero cells and the titre was found to be 10 4.6TCID50/ml indicating that chitosan encapsulation was able to maintain virus infectivity.
Citations |
 |
 |
 |
 |
