National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Objective of the research was to validate the potential of Syzygium aromaticum and Kaempheria galanga against biofilm formation and plasmid borne drug resistance exhibited by Pseudomonas spp. Antimicrobial property of S.aromaticum and K.galanga on P.aeruginosa culture was assessed using the agar well diffusion method. Crystal violet assay was employed to determine the percentage inhibition of P.aeruginosa biofilm by the extracts following generation of biofilm on multiwell plates. The decrease in Pseudomonas viability in an established biofilm after treatment was determined by standard MTT reduction assay. Fluorescent microscopy following staining with Ethidium bromide and Acridine orange was done to distinguish the dead and live cells in the biofilm. Finally, antiplasmid activity was established by agarose gel electrophoresis. The extracts were found to have potent antibacterial activity and inhibited biofilm attachment to polymeric surfaces. They further exhibited a significant decrease in biofilm bacterial cell viability when determined by MTT assay and fluorescent staining. S aromaticum effectively removed plasmids from Pseudomonas spp which can be due to their potential curing efficiency. The results obtained from the study validate the antibacterial and anti plasmid potential of extracts from Syzygium aromaticum and Kaempheria galanga against P.aeruginosa biofilm. The combination may be recommended as a potential drug to prevent biofilm formation and plasmid borne drug resistance.
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