National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Gladiolus (Gladiolus hybrida) is an important ornamental plant cultivated world over for its attractive spikes. The commercial cultivation of Gladiolus is based on natural multiplication of corms and cormels. In vitro propagation techniques, is superior to conventional propagation and produces disease free plants in huge quantities. In vitro regeneration of gladiolus cultivars, Sylvia, White Prosperity and Amsterdam was achieved using shoot bud of cormels as an explant. Sylvia variety recorded the best for callus induction and regeneration than other two varieties. The concentration of plant growth regulators affects the regeneration response differently. The highest callus induction rate of 76% explants was recorded on Murashige and Skoog (MS) medium supplemented with 2 mgl-1 2,4-D (2,4-Dichlorophenoxyacetic acid), after 28 days. MS medium supplemented with BAP at 2.0 mgl-1 exhibited higher shoot proliferating efficiency, i.e shoots per explant in Sylvia variety.
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