International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:7, July, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(7): 933-938

Molecular Characterization of Heat Tolerance Chickpea (Cicer arietinum L.) Genotypes using Random Amplified Polymorphic DNA (RAPD) Markers
Devendra Vasht1*, S.K. Dwivedi1, Yogendra Singh2, R.K. Samaiya1,A.  Babbar2, S.K. Pooniya3 and Vinod Kumar2
1Department of Plant Physiology
2Department of Plant Breeding & Genetics
3Department of Biotechnology, Jawaharlal Nehru KrishiVishwaVidyalaya, Jabalpur (MP), India
*Corresponding author

Chickpea (Cicer arietinum L.) is most important pulse crop of India in term of area and production and is extensively grown from human consumption mainly in South Asia and Middle East. Chickpea (Cicer arietinum L.) is a cool season legume crop and is grown in several countries worldwide as a food source. Molecular markers and variation in genomic DNA sequences have played a major role in varietal characterization and improvement of many crop species and have contributed to our abilities to assess biodiversity, reconstruct accurate phylogenetic relationships and transferring of particular traits. The polymorphism % ranged from 33.33 % (OPX-2) to 100% (OPB-6, OPX-4, OPB-12, OPA-17, OPA-11). The dendrogram was generated and 20 genotypes were aligned in 3 groups i.e. A has 10 genotypes, B has only one genotype and C has 9 genotypes. For molecular characterization of 20 genotypes, 10 RAPD primers showing polymorphism were used.

Keywords: DNA Isolation, RAPD primers, Polymorphism

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How to cite this article:

Devendra Vasht, S.K. Dwivedi, Yogendra Singh, R.K. Samaiya, A. Babbar, S.K. Pooniya and Vinod Kumar. 2019. Molecular Characterization of Heat Tolerance Chickpea (Cicer arietinum L.) Genotypes using Random Amplified Polymorphic DNA (RAPD) Markers.Int.J.Curr.Microbiol.App.Sci. 8(7): 933-938. doi:
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