Tomato leaf curl virus disease (ToLCVD) is one of the major causes for highest economical loss of tomato in India. In this study, Natural incidence of ToLCV disease on tomato was observed at Gujarat, India in the March, 2019. The infected plants revealed systemic viral symptoms in the form of sever leaf curling, leaf crumple with marginal yellowing, stem warped and stunted. Molecular methods were followed to further characterized coat protein (CP) gene of tomato leaf curl virus using specified primers. The purified PCR product (~800 bp) was sequenced and further analysed showed that the virus had 99.75% sequence identities and closest phylogenetic relationships with various isolates of Tomato leaf curl New Delhi virus, therefore name as Tomato leaf curl virus isolate NAU Gujarat coat protein (AV1) gene and submitted to NCBI (MK955892.1). ToLCV coat-protein sequence was computationally analysed to study evolutionally classification and further modelled to predict structure using swiss model that further validated using Ramachadran plot showing 89.10% amino acid in most favourable region. The present studied may be useful for development of diseases resistance approach for various crops against begomoviruses to minimize the yield losses.

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