National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The antimicrobial activity of Azadirachta indica leaf extract (Neem leaf) was carried out on Staphylococcus aureus, Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli using the agar well diffusion method. Ethanolic, aqueous and methanolic extracts of the plant were used at varying concentrations of 200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12.5 mg/ml, 6.25 mg/ml, 3.125 mg/ml and 1.56 mg/ml respectively. The three extracts were found to show a very high antimicrobial activity though the ethanolic extract possessed more activity than the methanolic and aqueous extracts. The minimum inhibitory concentration (MIC) was found to range from 1.56 mg/ml to 6.125for the three extracts respectively. When compared with standard antibiotics, the ethanolic and methanolic extracts showed maximum inhibition on these organisms. The phytochemical analyses revealed the presence of alkaloids, saponins, tannins, glycosides, steroids, terpenoids, and reducing sugars. Leaf extract of Azadirachta indica is therefore recommended for the treatment of human infections associated with these organisms.
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