National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Serological tests plays an important role in the diagnosis of leptospirosis as many clinical manifestations simulate diseases like dengue fever, hepatitis, Enteric fever and Malaria. Laborious and time consuming MAT, a gold standard test led to the introduction of Immunochromatography based tests, card tests and various ELISA kits for rapid diagnosis of acute leptospirosis by peripheral laboratories. The objective of our study was to know the efficacy of the tests like Leptocheck WB IgM(ICT), PanBio IgM ELISA(ELISA), with MAT. Hundred and eleven serum samples from 54 patients with clinical suspicion of leptospirosis and 50 serum from asymptomatic subjects was tested for IgM antibodies by the kits. Out of 54 serum collected, 42.59% were positive by ICT, 48.14% by ELISA, and 53.7% by MAT. ICT on comparison with MAT showed sensitivity of 75 % and 77.4%, Specificity of 96% and 95.6%, Positive Predictive value (PPV) 95.7% and 96%, Negative Predictive Value (NPV) 77.4% and 75.9% respectively and Kappa value 0.707.ELISA recorded a sensitivity of 89.7% and 90.3%, specificity of100%, PPV of 100%, NPV 89.3% and 88.46 respectively and Kappa value 0.889 with MAT. As ELISA was more sensitive and specific in picking up leptospiral cases early in the infection when compared to ICTs,thusshould be included with ICTs in laboratories to increase the detection of suspected cases of acute leptospirosis with history of 4-6 days of febrile illness.
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