National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The detection of extended- spectrum β-lactamase (ESBL) producing bacteria is important for infection control and epidemiological surveillance. The purpose of the present study was to compare two phenotypic methods for detection of ESBL positive Escherichia coli and Klebsiella pneumoniae in urinary isolates. This study was carried out in the Department of Microbiology, Rama Medical College, Kanpur(U.P), India from November 2015 to August 2016. Patients of all ages and genders were taken as study population. Lactose fermenting gram negative bacilli(GNB) were analyzed and routine susceptibility testing was performed. Screening test for ESBL production was done by two disc diffusion methods Ceftazidime and Ceftazidime+Clavulinic acid(CAZ/CAZC) and Cefotaxime and Cefotaxime+ Clavulinic acid (CTX/CTXC). In our study, 576 urine samples have been collected, from them 369 uropathogens were isolated 102 (17.07%) Escherichia coli, 66 (11.45%) Klebsiella pneumoniae and 201 others(34.89%) (including Staphylococcus aureus, CONS, Proteus species, Pseudomonas aeruginosa, Acinetobacter species and candida species) respectively. The prevalence of ESBL producing Escherichia coli and Klebsiella pneumoniae in our study is 30.39% and 36.36% respectively. Of the 65 Escherichia coli and 41 Klebsiella pneumoniae Screned For ESBL . 46 isolate were detected by combined disc method. The use of ceftazidime(30 µg) and ceftazidime + clavulanic acid(30µg/ 10µg) detected 46 isolates as ESBL positive as compared to cefotaxime(30 µg) and cefotaxime + clavulanic acid(30µg/ 10µg) which detected 33 isolates as ESBL positive. The use of ceftazidime(30 µg) and ceftazidime + clavulanic acid(30µg/ 10µg) DDDT phenotypic methods are useful diagnostic tool for detection of ESBL in gram negative bacilli..
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