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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
T A total of 23 putative Listeria isolates obtained from different sources, viz. food, animal, human, caterpillar and mosquito were screened for presence of the virulence factors by multiplex polymerase chain reaction (mPCR). Multiplex polymerase chain reaction for the amplification of isp and prs genes was employed for genus and species identification, while virulence profiling was employed by amplification of plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla genes. All strains harbours virulence genes plcA, hlyA, actA, prfA, inlC, inlJ, luxS and fla. Finally this study validated mPCR in the analysis and rapid detection and virulence profiling of L. monocytogenes. Irrespective of species of origin all the virulence genes are expressed by all isolates coequally.