National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of this study was to evaluate the effect of Tris-based extenders containing glycerol and egg yolk on the motility and viability of chilled ram semen collected during non-breeding season. Nine ejaculates from three healthy rams in non-breeding season were collected by artificial vagina method. The semen was diluted with Steridyl (ST), Triladyl (TRY) or Tris-based extender containing 5 % glycerol and 5% egg-yolk (TGGY) to a final concentration 200x109 spermper ml. Semen samples (n=9) for each extender were stored at 50C for 72 hours. Motility and viability were evaluated at 0, 6, 24, 48 and 72 h of storage. The results were statistically processed and the influence of extender and time of storage were recorded. After 6 h of storage the initial motility and viability did decrease (P<0.05) in all extenders but until 48 h significant differences among values for the same interval were not determined. At 72 h the values for ST and TGGY were increased (P<0.05) compared to TRY, but rather unsatisfactory. The increased time of storage was negatively correlated with both indicators in all extenders (P<0.05). In conclusion, Tris-based extenders containing glycerol and egg yolk demonstrated good protective effect on ram semen collected during the non-breeding season and stored at 50C. The type of extender had no influence on sperm motility and viability until 48 h of storage, while the time of storage significantly (P<0.05) affected the semen parameters.
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