National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A red pigment producing bacteria was isolated from soil sample which was identified by 16S r RNA sequencing as Serratia rubidaea JCM 1204T. The red pigment produced was tested for prodigiosin by presumptive color test, adsorption spectra, and its quantification was carried out on King’s B medium. For presumptive color test, the extracted pigment solution was divided into two portions. One part was acidified with a drop of concentrated HCl and the other part was alkalinized with a drop of concentrated ammonia solution. A red or pink color in the acidified solution and a yellow or tan color in the alkaline solution indicated a positive, presumptive test for prodigiosin. For adsorption spectra, the ethanolic acidified and alkalinized red pigment extract was tested by spectrophotometer in range between 300 to 700 nm and found that the ethanolic acidified red pigment extract absorbed maximum light at 535 nm while ethanolic alkalinized red pigment extract absorbed maximum light at 470 nm which is characteristic of prodigiosin. The solid red pigment was also further characterized by IR, NMR, and Mass spectrophotometer, which confirms that the red pigment produced by Serratia rubidaea JCM 1204T as prodigiosin.
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