Microscopic Colonization of Salt Tolerant Plant–Bacterium Microvirga zambiensis Strain WSM 3693 in Cotton Tissue using Auto Fluorescent Proteins GFP

R.A. Bhadania; M.V. Parkhiya; D.L. Akbari; L.F. Akbari; H.N. Bhalani; B.A. Golakiya

doi:https://doi.org/10.20546/ijcmas.2019.802.403

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Original Research Articles Volume : 8, Issue:2, February, 2019

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Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3449-3453

DOI: https://doi.org/10.20546/ijcmas.2019.802.403

Microscopic Colonization of Salt Tolerant Plant–Bacterium Microvirga zambiensis Strain WSM 3693 in Cotton Tissue using Auto Fluorescent Proteins GFP

R.A. Bhadania, M.V. Parkhiya, D.L. Akbari, L.F. Akbari, H.N. Bhalani and B.A. Golakiya

Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh 362001, India

^*Corresponding author

Abstract:

The Microvirga zambiensis strain WSM 3693 is an endophytic strain isolated from saline area of kutch, Gujarat. The green fluorescent protein (GFP) was used to mark this endophyte in order to visualize and analyze the colonization processes in vivo. After inoculation for 2 to 15 d, it was visualized in cotton tissue using fluorescence microscopy. Thus, the objectives of this study were to construct gfp-marked derivatives of Microvirga zambiensis strain WSM 3693and to determine the endophytic patterns of colonization of cotton under gnotobiotic conditions by using genetically marked derivatives of this strain.

Keywords: Microvirga zambiensis strain WSM 3693, Endophyte, GFP, Cotton

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How to cite this article:

Bhadania, R.A., M.V. Parkhiya, D.L. Akbari, L.F. Akbari, H.N. Bhalani and Golakiya, B.A. Microscopic Colonization of Salt Tolerant Plant–Bacterium Microvirga zambiensis Strain WSM 3693 in Cotton Tissue using Auto Fluorescent Proteins GFP.Int.J.Curr.Microbiol.App.Sci. 8(2): 3449-3453. doi: https://doi.org/10.20546/ijcmas.2019.802.403

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Original Research Articles Volume : 8, Issue:2, February, 2019

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