National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To evaluate the efficacy of NS1 antigen as a early marker for diagnosis of Dengue virus infection. Performance of NS1 antigen assay in comparison to MAC ELISA was evaluated when performed together in a single sample in Group 1. 391 acute/early convalescent sera were screened by both the assays. Group 2 evaluated the specificity of NS1 assay in comparison to MAC ELISA on 30 samples included as controls. In Group 1, 162(41.43%) were positive by NS1 antigen assay and 71(18.15%) were positive by MAC ELISA. NS1 antigen positivity decreased from 96.91% in acute phase sera to 3.08% in early convalescent sera (P < 0.0001). Conversely IgM positivity increased from 7.04% in acute phase sera to 92.95% in early convalescent sera (P < 0.0001). All the samples in Group 2 were negative showing 100% specificity of both the assays. NS1 Ag is an effective tool for the diagnosis of DENV infection, especially with in first 7 days of illness. Combined use of NS1 antigen assay with MAC ELISA test could significantly improve diagnostic sensitivity of dengue infection.
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