National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Response surface methodology (RSM) is a combination of statistical and mathematical techniques used to create the model and to analyze a response influenced by several factors. The present research was carried out to enhance bacteriocin production by the Lactic acid bacteria Lactobacillus gasseri NBL 18 isolated in our lab from infant fecal samples. The influence of physical parameters viz. temperature (37-42°C), pH (4.0-8.0), incubation time (6-24h) and inoculum level (1- 3%) on bacteriocin production was analyzed through RSM. Maximum bacteriocin production of 2.56 X 104 AU/ml was obtained at temperature 37°C, pH 8.0, inoculum size 3% and incubation time of 24 h. Statistical analysis showed that all the four factors had significant effects on bacteriocin production. RSM proved to be a powerful tool in the optimization of bacteriocin production by L. gasseri NBL 18.
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