National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was undertaken with an objective to develop and validate a panel containing maximum number of microsatellite markers that can be amplified in a single PCR reaction for precise parentage verification in Indian HF cattle population. The study was based on a total of 210 HF cattle (100 calf, 100 dam and 10 sires). Genomic DNA was extracted from blood and semen samples. A panel of 12 microsatellite markers (BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, ETH225, BM1818, ETH3, TGLA53) was amplified in a single multiplex reaction and analyzed by capillary electrophoresis on an automated DNA sequencer. The observed heterozygosity (Ho) of 12 markers ranged from 0.607 (TGLA53) to 0.904 (BM2113) while the expected heterozygosity (He) ranged from 0.581 (ETH225) to 0.873 (INRA23). Eleven out of 12 microsatellite loci revealed relatively high polymorphic information content (>0.6). The results suggest that multiplex microsatellite panel can be effectively used to verify the parentage as well as to assign the putative sire to daughters under progeny testing.
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