National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present studies were carried out at Agricultural Research station, Perumallapalle to establish an efficient system for in vitro regeneration of sugarcane cultivar, Co86032 using chemical mutagens; Ethyl methane sulphonate (EMS) and 2, 4-dichlorophenoxy acetic acid (2, 4 D). Young leaf rolls were used as explants for callus induction on MS medium containing different concentrations of EMS (0.6 µM l-1, 0.8 µM l-1 and 1.0 µM l-1) and 2, 4-D (4mg l-1, 5mg l-1 and 6 mg l-1). Among the different concentrations, Co86032 had taken minimum number of days (11.89) for callus induction with 2, 4 D @ 4mg l-1, recording maximum mean callus size of 2.19 cm with high callus induction frequency (96.30%) and maximum mean number of explants inducing callus (2.78) in addition with superior response in shooting, rooting and hardening characters than EMS.EMS and 2, 4 Dat higher concentration (1.0 µM l-1 and 6mg l-1, respectively) had inhibitory effect on callusing, shooting and rooting and produced morphological traits variation which can be exploited in breeding programs.
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