National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the present study, High Performance Liquid Chromatography with Ultra-Voilet detector (HPLC-UV) technique was standardized and validated for the detection and quantitation of quinolones antimicrobial residues viz. enrofloxacin, norfloxacin and ciprofloxacin from milk. The standardization procedure showed that the values for the system precision (% RSD) for both the analytes was 11% for area and <0.9% for retention time), linearity (r2>0.98), specificity and accuracy (70-110%) and precision (<10%) were within accepted range and demonstrated system suitability for analysis of milk samples. The standardized and validated method was applied for the detection of quinolones residues from 100 randomly milk samples collected from local market of Hisar (Haryana). Mean concentrations of norfloxacin and enrofloxacin antimicrobial residues in market milk samples were 3.54 and 2.02 µg/kg, respectively. A total of 8 samples were found to be containing quinolone antimicrobial residues. Comparison of antimicrobial concentration in each positive sample of milk with international MRLs showed that, none of the three antimicrobial was responsible for violations of set residue limits. It was concluded that milk is significant source of antimicrobial residues.
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