International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 8, Issue:2, February, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2696-2701

PCR Detection of blaIMP Gene in Metallo-β-Lactamase Resistant E. coli Isolated from Clinical Samples
Rahul Narasanna, Siddarth Surwonse, Manjunath Chavadi and Chandrakanth Kelmani*
Department of Biotechnology Gulbarga University Kalaburagi, India
*Corresponding author

PCR detection of blaIMP gene in Imipenem resistant E. coli is the aim ot this study. Total of n=66 of n=23 Imipenem resistant E. coli were selected for the present study based on the drug resistance pattern and their MIC to Imipenem. Phenotypic detection of MBL production was carried out and subsequently, PCR amplification of blaIMP was carried out. Among selected (n=23) Imipenem resistant E. coli (IREC), 100% (n=23) strains were positive for Imipenem mediated MBL production. Twenty-three MBL positive IREC isolates were shown presence of Plasmid DNA, with size measured about approx. 6kb. PCR amplification of blaIMP gene codes for Imipenem mediated MBL production yielded amplified blaIMP gene product with amplicon size of 189 bp. The presence blaIMP gene also contributes for MBL production which makes organisms to show reduced susceptibility against Imipenem antibiotic

Keywords: Imipenem resistant E. coli, MBL, blaIMP

Download this article as Download

How to cite this article:

Rahul Narasanna, Siddarth Surwonse, Manjunath Chavadi and Chandrakanth Kelmani. 2019. PCR Detection of blaIMP Gene in Metallo-β-Lactamase Resistant E. coli Isolated from Clinical Samples.Int.J.Curr.Microbiol.App.Sci. 8(2): 2696-2701. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.