National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Niger [Guizotia abyssinica (L.f.) Cass.] is one of the important minor oilseed crops in India. Phyllody disease on niger noticed for the first time at Main Research Station, Hebbal, Bengaluru, Karnataka. The molecular detection and characterization of the phytoplasma causing niger phyllody was investigated during Kharif 2016. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by polymerase chain reaction (PCR) from infected samples, as well as by sequencing and phylogenetic analysis. The molecular detection by using nested PCR phytoplasma specific universal primers R16F2n/R16FR2 revealed the amplification of phytoplasmal specific PCR product of 1.2 kb fragment corresponding to the 16S rDNA. The16S rDNA sequence of niger phyllody phytoplasma had maximum nucleotide identity of 90 per cent with the 16S rDNA sequence of Cymbopogon citratus white leaf phytoplasma (KF773150.1), Alfalfa witches'-broom Phytoplasma Mes 38(KT943964.1), Sesamum indicum phyllody Phytoplasma (KY547787.1) and Vigna radiata phyllody NDL (KY439871.1).
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