National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Papaya ring spot virus (PRSV) is the most destructive disease of Papaya, responsible for yield reduction of Papaya all over the world. PRSV exhibited different types of symptoms on Papaya plant in the field like ringspot , vein clearing, vein banding, chlorosis of younger leaves, filiformy, shoestring, mosaic, mottling, stunted growth etc. Molecular diagnosis of PRSV was done with the help of gene specific primers through PCR. Gel electrophoresis results show a 300bp clear band which confirms the presence of PRSV in the plants. Sequencing was done and phylogenetic tree was made. Sequencing results showed 89-97 percent identities with other PRSV isolates present in NCBI Genebank. Phylogenetic analysis reveals that the PRSV Jorhat isolate is closely similar to that Assam isolate Accession no. KC149500.
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