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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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Original Research Articles                      Volume : 8, Issue:1, January, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(1): 1351-1368
DOI: https://doi.org/10.20546/ijcmas.2019.801.144


Cryopreservation of Buffalo Bull Semen- Restriction and Expectation: A Review
Pavan Kumar Mittal1*, A.K. Madan3, Vijay Sharma2, G.S.Gottam1 and Barkha Gupta1
1Department of Veterinary Physiology and Biochemistry
2Department of Veterinary Public Health and Epidemiology, Post Graduate Institute of Veterinary Education and Research, Jaipur, Rajasthan-302031, India
3Department of Veterinary Physiology, College of Veterinary and Animal Sciences, DUVASU, Mathura-281001, India
*Corresponding author
Abstract:

Semen cryopreservation is a widely used technique across the world for conservation and proliferation of genetically superior germplasm through artificial insemination. Artificial insemination (AI) is the most conclusive tool to increase genetic improvement, control of venereal diseases and improve herd performance and productivity. For achieving the mission, successful and effective cryopreservation of semen is essential. In recent years, increased demand of animal products due to very rapid growth of human population inspired the investigators to increase per animal milk production using various techniques of Biotechnology. The process of semen cryopreservation is stressful and around 40%-50% of sperm lose their motility and viability due to loss of structural and functional capabilities. One of the factors which affect post thaw survival of sperm is the freezing rate. In buffalo, poor fertility rate with cryopreserved semen is main obstacle in its proliferation. It is need of the time to spread the knowledge of various factors and components such as use of suitable extenders, additives, cryoprotectants, freezing and thawing protocols contributing in cryopreservation of semen to improve the fertility rate of buffalo.


Keywords: Buffalo, Buffer, Cryopreservation, Cryoprotectant, Extender, Semen Processing, Semen

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How to cite this article:

Pavan Kumar Mittal, A.K. Madan, Vijay Sharma, G.S.Gottam and Barkha Gupta. 2019. Cryopreservation of Buffalo Bull Semen- Restriction and Expectation: A Review.Int.J.Curr.Microbiol.App.Sci. 8(1): 1351-1368. doi: https://doi.org/10.20546/ijcmas.2019.801.144
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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