National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A clinical investigation was conducted with the objective to isolate and identify the uterine aerobic bacteria in post partum crossbred dairy cows suffering from metritis and antibiotic sensitivity testing using disc diffusion test. Fourteen post partum crossbred dairy cows found positive for clinical metritis at Research cum Development Project (RCDP) on Cattle, Mahatma Phule Krishi Vidyapeeth (MPKV), Rahuri Maharashtra State were investigated in the present study. The biochemical characterization results indicated that E. coli was isolated from 4 samples; while 3 each sample was found positive for Klebsiella spp. and Staphylococcus spp. From the remaining 4 metritic samples, no growth was observed either on MacConkey or blood agar. Antibiotic sensitivity pattern revealed that highest resistance was observed in Ampicillin and Amoxycillin (90.0% each), followed by Co-trimoxazole and Enrofloxacin (70.0% each), Tetracycline (50.0%), Ceftriaxone and Gentamicin (40.0% each). While all the isolates were found sensitive to Chloramphenicol and Ciprofloxacin antibiotics. Multiple drug resistance was observed in 7 isolates. Earlier research findings at RCDP on Cattle, MPKV, Rahuri revealed that Enrofloxacin was the drug of choice for treatment of E. coli associated Metritis. But present indicated the development of resistance of E. coli to Enrofloxacin antibiotic. The paradigm shift in resistance pattern may be due to consistent use of Enrofloxacin for treatment of Metritis resulting into development of resistant mutants.
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