National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The cellulolytic efficiency of yeast isolates obtained from different fruit and fruit wastes was evaluated in terms of degradation of cellulose, release of reducing sugars and bioethanol production. The isolates CY-3, CY-4, CY-8 and CY-15 together with a reference cellulolytic strain Saccharomyces cerevisiae (NCIM 3220) displayed maximum hydrolysis of carboxy methyl cellulose (CMC) to yield reducing sugars when determined in 2 days interval for up to 22 days, Based on this, the isolates were further evaluated for combined saccharification and fermentation of a pretreated lignocellulosic substrate such as wheat straw in flask culture studies. The concentration of ethanol produced by the cellulolytic yeasts at the end of fermentation was determined by Gas Chromatography. Strain CY-15 produced 0.573g/L of ethanol which was on par with the reference strain which produced 0.68 g/L. Thus the study attempts to develop a sustainable bioethanol production technology by simultaneous saccharification and fermentation of wheat straw using native yeasts.
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