National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A set of sixty microsatellite markers were used to characterize 49 genotypes of wheat. Out of these, 23 markers showed polymorphism with a total of 170 alleles. Average number of SSR alleles per locus was 2.88 and ranges from 2 (Cfa2040, Xgwm261, Xgwm382, Xgwm413, Xgwm170, Wmx44, Cslv34, Xgwm501, Xgwm70, Barc136, Barc101, Xgwm352, Xgwm130, Barc187, Barc76, Wmc407) to 5 (Xgwm190). The PIC value varied from 0.11-0.65 and 7 out of 23 SSR loci, Xgwm261, Xgwm295, Xgwm190, Xgwm46, Gwm11, Xgwm273 and Xsps3000 had PIC value more than 0.5 thus considered very useful in estimating genetic diversity of the genotypes. The UPGMA cluster grouped into two, major cluster 1 consists of 6 sub clusters whereas major cluster 2 was not further fragmented and had only one genotype WH1191. Cluster pattern revealed that, sub-cluster 2 was the largest consisting maximum number of 24 genotypes. This cluster analysis grouped genotypes on the basis of similarity at DNA level, thus for selecting parents for hybridization program against yellow rust disease can be chosen from different clusters.
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