National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was carried out for the detection of Avibacterium paragallinarum from Avian Infectious Coryza suspected poultry carcasses of Jabalpur region. The polymerase chain reaction (PCR) was standardized for the diagnosis of Infectious Coryza by using live Avibacterium paragallinarum broth containing Modesto, Spross and 0083 strains received from Stallen South Asia Private Limited, Mumbai, as source of DNA of A. paragallinarum. Poultry carcasses (n=60) showing characteristic gross lesions of Avian Infectious Coryza were investigated in the present study. Samples were collected from the infra orbital sinus, nasal cavity and trachea of the entire sixty carcass. The swabs of the three different sites from one bird were mixed and then pooled sixty samples were tested by PCR using oligo primer specific to 16S rRNA of Avibacterium paragallinarum. PCR analysis revealed 08 positives (13.33%) for Avian Infectious coryza out of total 60 samples tested. It is concluded that PCR an early, rapid, accurate and highly sensitive diagnostic technique with the ability to substitute conventional cultural examination can be used to prevent the economic losses associated with the disease.
Citations |
 |
 |
 |
 |
