National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Identification of alleles of a gene and then utilizing them in crop improvement programme is the main objective of any plant breeder. A breeding programme cannot exist if there is no variability. For the screening of germplasm for a desired allele, breeders need large area, labour and fund. Also this screening is amenable to environmental effect. Target Induced Local Lesions In Genome (TILLING) is a perfect solution of these problem. TILLING is a reverse genetics approach which uses mutagens to create noval variability in a particular allele. This variability in a gene is then screened using various sequencers to find out various mutants. The best mutant of them is then can be used in breeding programmes directly. In a modified version, natural population is screened to identify all alleles of a gene. This new version of this technique is called EcoTILLING. Using these reverse genetics approaches, a large number of genes are identified and used in breeding of various crop species.
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