National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The experiment was conducted to study the effect of nature of explants and its orientation on shoot establishment over MS media in Kufri Bahar and Kufri Surya cultivars of potato under in vitro conditions. The results indicated that a combination of 0.4% Bavistin (25 min) + 0.1% HgCl2 (30 sec) provided the maximum number of aseptic explants (73.33%). The results have shown that in Kufri Bahar 69.18 per cent sprouts produced shoots with higher number of shoots/explant. While in case of Kufri Surya sprout’s bits responded very well (90.00%) and sprouts have produced more number of shoots/explant (6.00), average number of nodes/explants (5.12) and average shoot length (6.38 cm). The results have further shown that orienting of explants vertically, significantly increased the per cent establishment of explants (75.68% in Kufri Bahar and 70.78% in Kufri Surya), whereas, the average number of shoots, average number of nodes per explants and average shoot length was found to be more in case of horizontal orientation of explants over the media.
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