National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was designed to isolate and determine the antibiotic sensitivity pattern of bacteria associated with the hands of commuters in Akure metropolis. The samples were collected using sterile swab sticks at different motor parks (Obaile, Oke-Ijebu, Benin, FUTA and Road block), then transported to Microbiology laboratory for further analyses. Isolation of microorganisms was carried by direct plating on the bacteriological media. The plates were incubated at 37oC for 24 hours. Enumeration of the bacterial isolates was done by direct count of the colonies formed on the plates. Pure bacterial isolates was obtained by repeated streaking on the fresh media. Different bacteria were isolated. Staphylococcus aureus recorded high frequency (90%) while Escherichia coli recorded least frequency (12%). All the Gram negative bacterial isolates were susceptible to some of the antibiotics with varied zone of inhibition. Shigella spp was susceptible to ciprofloxacin with zone of inhibition 5.0mm. Escherichia coli and Staphylococcus aureus were resistant to pefloxacin. High microbial loads recorded from the commuters indicated the level of contamination. Therefore, hand sanitization, proper hygiene and the use of antibiotics could help in the control and spread of antibiotic resistant bacteria from fecal origin.
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