|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Blackgram (Vigna mungo) is a major pulse crop grown in Tamil Nadu and leaf crinkle virus is an important disease that infects the crops at various stages of its growth, which reduce the yield drastically. There are conflicting reports on the transmission of ULCV by insects that includes aphids, leafhopper, whitefly and beetle. Hence, a study was carried out to identify the putative vectors involved in the transmission of the disease. Found that the virus was effectively transmitted by aphid vector, Aphis craccivora in a non-persistent manner and whitefly (Bemisia tabaci). No transmission was observed by melon aphid (Aphis gossypii), cabbage aphid (Brevicoryne brassicae) and hadda beetles (Epilachna vignitioctopuntata and Epilacna dodecastigma). The per cent transmission by aphids was high compared to whiteflies to extent of 83.3 and 66.6 per cent respectively. Virus-vector relationship revealed that minimum of 5 adults of A. craccivora with an acquisition access period of 1 minute and inoculation access of 5 minutes could transmit the ULCV. However, maximum transmission (83.3%) was obtained by 10 aphids/plant with an acquisition access period of 5 to 10 minutes, inoculation feeding of 10 minutes. Minimum of five whiteflies were required to transmit the ULCV. However, sixty six per cent transmission of ULCV disease was obtained when 10 whiteflies were released per healthy plant. Acquisition access period of 36 to 48 h and inoculation period of 24h results in increased in transmission up to 66 per cent. Serial transmission up to 56 h results upto 50 per cent transmission. Beyond 72 hours no transmission of disease was observed.