National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was designed to study the prevalence and characterization of shiga toxin producing Escherichia coli from dairy animals, water samples in dairy farms and from humans who were in close contact with animals. A total of 508 faecal samples from dairy animals, 4 water samples from dairy farms and 28 Human faecal samples, who were in close contact with animals were subjected to cultural and biochemical tests for confirmation of E. coli. The molecular characterization of positive isolates with stx1, stx2, eaeA and hlyA genes and multiplex PCR for confirmation. A total 522 samples were positive for E. coli out of 540 (96.67%) samples by culture method, 319 (61.11%) positive on blood agar plate assay and various biochemical characteristics were studied. Out of 522 positive E. coli, 56 (10.72%) are positive for STEC by PCR method. On multiplex PCR of stx1, stx2, eaeA and hlyA genes 6 were positive 6 out of 56 (8.92%). The frequent isolation of STEC strains from non human sources like animals, food and other products along with the identification of multidrug resistance and virulence genes across the Indian subcontinent poses a serious threat of the outbreaks in humans that can occur in the future.
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