National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Mycobacterium avium subspecies paratuberculosis (MAP) is a Gram positive, acid-fast bacterium which causes Johne’s disease or Paratuberculosis in domestic livestock species. For screening of cows for MAP infection a total of 103 milk samples from individual cows were collected from organized (57) and unorganized (46) farms. Presence of anti-MAP antibodies in milk were screened by indirect enzyme linked immunosorbent assays (iELISA) test, 72 (69.90%) cow milk samples were found positives (66.07% from organized farms and 74.46% from unorganized farms). Positivity percentages were 62.50 in Gir cows and 70.52 in non-descriptive and crossbred cows irrespective of the farming system for the presence of MAP antibodies. Milk samples from 13 clinically suspected cows were used to prepare smears on glass slides and stained by Ziehl-Neelsen method. Microscopically 2 cow milk samples were found positive for presence of acid-fast organisms. Extracted nucleic acid, deoxyribose nucleic acid (DNA) from strong positive milk sample in microscopy was further tested in polymerase chain reaction (PCR) to amplify MAP DNA using insertion sequence 900 (IS900) specific primers. Except in positive control, specific amplifications were not visualized for test samples after agar gel electrophoresis. The present study indicates circulation of Mycobacterium avium subspecies paratuberculosis in cattle population of Mhow of Indore in Madhya Pradesh.
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