National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Salmonella enterica subsp. enterica serovar typhimurium is a gram-negative serious contagious pathogen having a broad host range. It has been associated with a wide spectrum of infectious diseases, including typhoid fever and nontyphoidal salmonellosis, which cause public health problems worldwide. Several genes contribute in virulence and determine pathogenicity of this isolate. inv E is an important invasion gene, member of Salmonella pathogenicity island1 (SPI-1) which helps pathogen to invade the host intestinal epithelial cell and also mediates the translocation of several bacterial effector proteins that have the capacity to stimulate cellular responses leading to bacterial internalization and the reprogramming of gene expression in the infected cells. In present study inv E gene was cloned, sequenced and phylogenetic analysis was done using bioinformatics approach. Genomic DNA was isolated from field isolate of Salmonella Typhimurium (A-201) and inv E gene was amplified using gene specific primers and cloned in pJET vector by the positive selection system. Amplification of inv E gene yielded a product of approximately 1.1 kb. Subsequently gene was sequenced and a complete ORF of 1119 bp was obtained. The sequence was submitted to NCBI Genbank and Accession No. KC607873 was allocated. The sequence was analysed using bioinformatics tools for the homologous sequences. Sequence homology was searched at nucleotide level and exhibited 99% similarity with Salmonella enterica strain FORC030 complete genome and Salmonella enterica subsp. enterica serovar Indiana strain D90, complete genome.
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