Diagnostic imaging allows visual evaluation of normal and abnormal morphology of the region and thus helps in pinpoint diagnosis of many diseases. But to identify the abnormality one has to be familiar with the normal anatomy of the system. Duodenoscopy of 60 clinically healthy dogs were performed by esogastrointestinal fiberscope and the appearance such as color of mucous membrane, presence of erythema, erosion and sub mucosal blood vessels on duodenal wall were evaluated. In procedure, the insertion tube was first passed directly into the duodenum through pylorus before actual examination of stomach. In order to pass the tip of scope in the pylorus, the pylorus was first fix carefully at the centre of endoscopic field and a gentle pressure was applied with rotation of both knobs simultaneously in counter clockwise direction. The color of mucosa of duodenum varied from pinkish red (38 cases) to yellowish red (22 cases) and it had velvety texture. Bile was noted in the duodenum in 16 cases, while sub mucosal blood vessels were visualized in two cases. However, erythema or erosion was not seen in any of the cases in this study.The mean distance of Junction of descending and ascending duodenum from upper incisor was measured as 69.15 ± 1.39, 100.85 ± 0.56 and 103.85 ± 0.51cm in small, medium and large sized dogs, respectively. On the basis of observations it could be said that working length of 100 and 110 cm would be suitable for small and medium sized dogs, respectively. The use of esophagogastroduodenoscope with working length of 110 to 120 cm would be more appropriate for large sized dogs.

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