National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A field survey was conducted in major tomato growing districts of Tamil Nadu viz., Coimbatore, Dharmapuri, Krishnagiri, Salem and Madurai during 2015-2016. Groundnut bud necrosis virus (GBNV) infection on tomato exhibited the symptoms viz., chlorotic and necrotic spots on leaves, necrosis of growing young buds (bud blight), bronzing, wilting and stunting. The per cent disease incidence of GBNV ranged from 18.6% to 35.3% in the above districts. A high per cent disease incidence of 35.3% was recorded in Thondamuthur village of Coimbatore district. Cowpea cv. CO5 was used as local lesion host for maintenance of GBNV isolates separately. A DNA fragment of around 800bp corresponding to a part of replicase gene was amplified using the tospovirus universal primer pair in all the samples. The DNA fragments were cloned into pGEMT vector and sequenced. The sequence analysis revealed higher similarity among the present isolates. However, they had an identity of 92.5% to 99.3% with the other isolates in NCBI. The results revealed that the isolates of GBNV is not divergent throughout Tamil Nadu.
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