National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A systematic and comparative study on mutagenic ability of different doses of ethyl methane sulphonate (EMS, an alkylating agent), gamma rays (an ionizing radiation) and EMS + gamma rays was carried out in a well-adapted desi chickpea variety HPG17. All mutagenic treatments were effective in inducing genetic variability. A proportional decrease in germination of mutagen treated seeds as well as subsequent plant survival was observed with increase in mutagen dose. Not only higher doses but combined treatments having low doses of EMS + gamma rays also reduced seed germination and plant survival with highest reduction in seed germination at 200 Gy + 0.05% EMS and in plant survival at 150 Gy + 0.05%. EMS appears to be better than gamma rays in induction of macromutations as maximum frequency of macromutants was at EMS (0.10%). Mutagenic effectiveness and efficiency was also studied to identify the most effective mutagen treatment. Overall EMS was more effective than the gamma rays and combination treatments as maximum effectiveness was observed at 0.05% and 0.10% EMS, respectively. The 300 Gy + 0.05% EMS had the highest mutagenic efficiency.
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