National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Tuberculosis is an important public health problem in India and globally. Prompt detection and susceptibility testing of Mycobacterium tuberculosis complex from clinical specimens is essential for appropriate management of patients with tuberculosis. With this in background, this study aimed at the comparison of isolation of Mycobacterium tuberculosis complex from clinical specimens of patients suspected of pulmonary tuberculosis using BACTEC Micro MGIT and Lowenstein Jensen (LJ) media with detection of resistance. A prospective study was carried out for one year from November 2016 to October 2017 in the Department of Microbiology, Pushpagiri Medical College, Tiruvalla, Kerala. A total of 81 samples were processed for Ziehl-Neelsen (Z-N) staining and was then cultured using liquid Mycobacterium growth indicator tube (BDTM BACTECTM Micro MGIT) and solid (Lowenstein Jensen’s) media. Isolates confirmed as Mycobacterium tuberculosis complex were subjected to drug susceptibility testing (DST) using modified proportion method by MGIT for 1st line drugs i.e. streptomycin (1.0µg/ml), isoniazid (0.1µg/ml), rifampicin (1µg/ml), ethambutol (5µg/ml) and pyrazinamide (100µg/ml). Of the total 81 samples that were cultured, seven (8.6%), were positive by both MGIT and Lowenstein Jensen’s. Three (3.7%) were positive by MGIT only and not by LJ. Only 4/10 (4.9%) culture positive samples were positive by direct smear examination. Turnaround times for culture positivity by MGIT vs LJ was an average of 15.1 days vs 23.8 days. Sensitivity to all drugs was observed in 4/10 (40%) isolates. None of these 10 isolates were multidrug resistant. Culturing of the organism in addition to smear, increases the possibility of microbiological diagnosis and offers a possibility for drug susceptibility testing.
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