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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:11, November, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 315-320
DOI: https://doi.org/10.20546/ijcmas.2018.711.039


Biochemical Composition of Different Straw Substrates and Activity of Cellulolytic Enzymes Produced by Pleurotus florida under in vitro Conditions
R.L. Sharma* and M.P. Thakur
Krishi Vigyan Kendra, Raipur, India Indira Gandhi Krishi Vishwavidyalaya, Raipur 492 012 Chhattisgarh, India
*Corresponding author
Abstract:

The present investigation was undertaken with the objectives to study the biochemical composition of Pleurotus florida grown on different straw substrates and activity of cellulolytic enzymes produced by Pleurotus florida under in vitro conditions. The results revealed that, the biochemical composition of P. florida raised on three straw substrates exhibited higher percentage of fat (2.33%) on soybean straw followed by wheat (1.74%) and paddy straw substrate (1.65%) on dry weight basis. The content of carbohydrate in P. florida was significantly higher when it was raised on wheat straw substrate (32.54%). Moisture (91.68-97.78%), ash (6.81-7.84%) and protein (18.30-20.0%) content of P. florida did not vary significantly with the different substrates used. The maximum activity of cellulase was observed in 20 and 16 days old culture filtrate of P. florida while, the activity was minimum in 8 days old culture filtrate. Cellulase production was maximum in acidic medium (pH 4.0) as compared to neutral (7.0 pH) and alkaline (9.2 pH) medium.


Keywords: Biochemical composition, Different straw substrates, Fat per cent, Carbohydrate content, Moisture (%), Protein content, Pleurotus florida

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How to cite this article:

Sharma, R.L. and Thakur, M.P. 2018. Biochemical Composition of Different Straw Substrates and Activity of Cellulolytic Enzymes Produced by Pleurotus florida under in vitro Conditions.Int.J.Curr.Microbiol.App.Sci. 7(11): 315-320. doi: https://doi.org/10.20546/ijcmas.2018.711.039
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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