'' '' '' '' '' Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (<em>Arachis hypogaea</em> sp. Virginia) at Flower Development Stage
Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
Home Editorial Board Current Issue Special Issue Archives Indexing Submit Article
Instruction to Authors Manuscript Charges Copyright Form Model Manuscript Process Flow (Time Frame) FAQ
Reviewer Guidelines Selection of Reviewers
Editorial Review Policy Embargo Policy Ethical Expectations Consent For Identifying Information Informed Consent Manuscript Withdrawal Policy Peer Review Process Plagiarism Policy Ethical Violations Appeal Policy Against Editorial Decision Advertising Policies Authorship Policy
Access Policies Authorship Publication Policie Publication Ethics Complaints Policy Confidential Information Conflicts of Interest Policy Consent for Copyrighted Material Copyright Policy Corrections and Retractions Digital Preservation Policy Research On Human And Animal Studies
Publication Ethics Contact
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Join as a Reviewer

Login as a Reviewer

Indexed in


National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here
Announcement

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 7, Issue:10, October, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 3524-3531
DOI: https://doi.org/10.20546/ijcmas.2018.710.407


Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage
Nimita Umretiya1*, Hitesh Jasani2, Nilam Tulsani1, Abhijeeta Nandha1 and B.A. Golakiya1
1Department of Biotechnology, Junagadh Agricultural University, Junagadh-362001, Gujarat, India
2College of Computer, Science and Information Technology,
Junagadh-362015, Gujarat, India
*Corresponding author
Abstract:

Protein is play critical role in flower development. Profiles of total flower development proteins isolated from different stages (Bud, Flower and Peg) peanut cultivar GJG-22, were studied using two-dimensional gel electrophoresis. Protein is extracted by trichloro acetic acid/acetone precipitation method. Comparative analysis was carried out of spots identified using platinum master software at three different flower stages. Protein revealed that total 9193 protein spots with the pH range 4 - 7 and 23-312 kDa range recorded. Out of 9193 spots, maximum 3413 spots were found in pH range 6 - 7 in groundnut samples. Proteomic analysis reveals the translational products of gene expression of plant during flowering stage.


Keywords: Two-Dimensional Electrophoresis (2-DE), Protein profile, Groundnut, Arachis hypogaea, Protein extraction
Download this article as Download

How to cite this article:

Nimita Umretiya, Hitesh Jasani, Nilam Tulsani, Abhijeeta Nandha and Golakiya, B.A. 2018. Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage.Int.J.Curr.Microbiol.App.Sci. 7(10): 3524-3531. doi: https://doi.org/10.20546/ijcmas.2018.710.407
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.
Home | Terms of Use | Copyright Form 2012-2020 Copy rights reserved IJCMAS. Powered by ThinkNEXT Technologies