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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2023: 95.56
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56
For more details click here
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Original Research Articles                      Volume : 7, Issue:10, October, 2018
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2018.7(10): 3524-3531
DOI: https://doi.org/10.20546/ijcmas.2018.710.407


Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage
Nimita Umretiya1*, Hitesh Jasani2, Nilam Tulsani1, Abhijeeta Nandha1 and B.A. Golakiya1
1Department of Biotechnology, Junagadh Agricultural University, Junagadh-362001, Gujarat, India
2College of Computer, Science and Information Technology,
Junagadh-362015, Gujarat, India
*Corresponding author
Abstract:

Protein is play critical role in flower development. Profiles of total flower development proteins isolated from different stages (Bud, Flower and Peg) peanut cultivar GJG-22, were studied using two-dimensional gel electrophoresis. Protein is extracted by trichloro acetic acid/acetone precipitation method. Comparative analysis was carried out of spots identified using platinum master software at three different flower stages. Protein revealed that total 9193 protein spots with the pH range 4 - 7 and 23-312 kDa range recorded. Out of 9193 spots, maximum 3413 spots were found in pH range 6 - 7 in groundnut samples. Proteomic analysis reveals the translational products of gene expression of plant during flowering stage.


Keywords: Two-Dimensional Electrophoresis (2-DE), Protein profile, Groundnut, Arachis hypogaea, Protein extraction

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How to cite this article:

Nimita Umretiya, Hitesh Jasani, Nilam Tulsani, Abhijeeta Nandha and Golakiya, B.A. 2018. Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage.Int.J.Curr.Microbiol.App.Sci. 7(10): 3524-3531. doi: https://doi.org/10.20546/ijcmas.2018.710.407
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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