National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Growing incidence of β-lactam resistance mediated by Extended Spectrum β-Lactamases (ESBLs) and Metallo-β-lactamases (MBLs) is a major concern due to their extreme resistance towards the commonly used antibiotics. The present study was undertaken to determine the in-vitro effect of antibiotic encapsulated micro/multiple emulsions on clinical isolates of 68 ESBL and 7 MBL-producing uropathogens by agar diffusion method. The water in oil (w/o) microemulsions of amoxicillin (particle size 11-15nm) and ceftazidime (particle size 17-19nm) did not exhibit antibacterial activity. However, water in oil in water (w/o/w) multiple-emulsion of both the drugs exhibited activity. Further, the effect of glyceryl dilaurate (GDL), a lipid emulgent having inherent antibacterial activity was studied. Ceftazidime multiple emulsions, with and without GDL showed zones of inhibition against all test uropathogens in the range of 11-32mm. Amoxicillin w/o/w multiple-emulsion without GDL was found to be ineffective. However, in presence of GDL, comparatively better activity was observed with zones of inhibition in the range of 10-13mm. All micro/multiple emulsions showed a PDI (Poly Dispersity Index) between 0.1-0.3. This study hereby indicates the potential of antibiotic micro/multiple emulsions to be used effectively for intracellular targeting.
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