National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Textile effluent polluted soil samples collected from the contaminated sites of Common Effluent Treatment Plant (CETP), Perundurai, Tamil Nadu, India were screened for isolation of potential bacteria capable of decolorizing commonly used textile azo dyes. A novel bacterium, designated as ‘Soil Isolate 01’ (SI 01) isolated from the contaminated site was able to efficiently decolourize all the chosen four azo dyes (Reactive Yellow 180, Reactive Red 180, Reactive Red 198 and Red RR) under optimized aerobic - microaerophilic condition in nutrient broth with pH 8 at 37oC and a dye concentration of 100 mg.l-1 with decolourization percentage > 85% in all the four dyes at 48 hrs. The biodegradation of the four azo dyes was monitored by UV–Vis analysis. Experiments revealed that the autochthonous bacterium SI 01 decolorized all the four azo dyes in such a way that its absorption peaks in the visible range disappeared indicating molecular re-arrangement of the dye structure. Scanning Electron Microscopic (SEM) analysis and Live versus inactivated cells studies proved that only live bacterial cells were able to decolorize the dye whereas inactivated cells could not is an evident that decolorization was not due to physical adsorption.
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