The antioxidant and antimicrobial properties of fish protein hydrolysate (FPH) prepared from Tilapia waste was evaluated. The Protein hydrolysates was prepared from tilapia (Oreochromis niloticus) waste using papain enzyme under the optimum conditions viz., temperature of 50°C, pH of 6.5, E/S ratio of 1% and 60 minutes time. The prepared proteinhydrolysate was evaluated for antioxidant and antimicrobial properties in vitro at different concentrations. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of FPH was highest at 100 mg/L (77.40%) and the maximum metal chelating activity of FPH was seen at 500mg/L (49.27%). The DPPH radical scavenging activity and metal chelating activity showed concentration dependent activity. FPH showed ferric reducing activity at all concentrations and exhibited highest reducing power at 500 µg /mL (2.18%). The antibacterial activity of FPH was seen at a concentration of 8 and 10 mg /mL FPH was potentially active against gram+ve bacteria viz., Staphylococcus aureus and Bacillus subtilis whereas, it showed smaller zones of inhibition against gram-ve Escherichia coli bacteria.

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